Agilent Platform
Asuragen is a Certified Service Provider for Agilent microRNA and aCGH microarrays.
Services Available
Features of the Agilent Microarray Platform:
Agilent microarrays are manufactured with high-performance SurePrint Technology™.
The arrays are manufactured using a non-contact in situ synthesis process of printing
40-60-mer length oligonucleotide probes directly onto the arrays, base-by-base,
from digital sequence files.
Agilent has developed a microarray-based application for studying microRNAs (miRNAs)
that combines a unique miRNA direct labeling method with an innovative probe design
and established high-performance SurePrint inkjet synthesis technology. The creation
of complete miRNA expression profiles using robust and highly sensitive microarrays
allows to gain broad insight into human, mouse, or rat miRNA expression and
regulation. This capability offers a unique opportunity to develop a confident and
clear picture of the intricate expression networks and systems that impact your
genomics research.
General specifications of the Agilent miRNA microarrays:
- 8-plex format (8 arrays per slide)
- 60-mer probe length
- 16-20 replicate features per miRNA
- current Sanger miRBase content for human, mouse, or rat
- direct end-labeling of total RNA using Cyanine 3 pCp
Microarray-based Comparative Genomic Hybridization (aCGH) is a powerful technique
used to identify and characterize DNA copy number variations (CNV) across the genome.
Agilent’s oligonucleotide aCGH platform is an integrated solution that gives you
the resolution and flexibility to perform genome-wide as well as customized zoom-in
profiling of genomic aberrations on a single chip, along with simplified experimental
design and ease-of-use protocols to handle genomic samples with full complexity.
The Agilent CGH platform consists of flexible multi-pack formats for both catalog
and custom arrays and enables copy number analysis of most sample types, including
FFPE, using the robust DNA Analytics analysis tool. The quality, flexibility, and
complete workflow combine to make Agilent CGH microarrays the highest performing
copy number solution.
General specifications of the Agilent aCGH microarrays:
- several flexible formats
- 60-mer probe length
- 1000 Replicated Biological Features (X3)
- currently available for human, mouse, or rat
- feature size ~65 microns
Learn more about Agilent Microarray Technologies
Asuragen utilizes the Agilent DNA Microarray Hybridization Oven, SureHyb Hybridization
Chamber, and Feature Extraction software.
Quality Control
No matter how robust the downstream processes, good data is dependent on sample
quality. Consequently, accurate measurement of sample quality is essential before
deciding if a sample merits further analysis. All samples prepared by Asuragen Services
are routinely qualified. Due to the partially degraded nature of nucleic acids from FFPE tissues,
special quality control measurements are used.
Yield:
Nucleic acid yield is primarily dependent on the tissue type and mass (or volume)
but is often affected by disease status, chemical treatment, media, preservatives,
and tissue handling.
Integrity:
RNA integrity is dependent on a wide range of factors in the precise handling of
biological materials from collection to RNA isolation. Although DNA is routinely
stable in most sample types, formalin fixation does lead to partial DNA degradation in FFPE
samples. For this reason, Asuragen offers a wide variety of services compatible
with RNA and DNA from FFPE.
Asuragen’s rigorous procedures enable Asuragen to be the only service provider to
offer a Data Quality Guarantee.
If, for any reason, a qualified sample does not pass our process control specifications,
we will re-run the sample at no additional charge.
Read more about our
commitment to the best possible data quality, or read about our contributions to
in-process controls and
standardization.
Sample Handling and Processing
All samples are received and entered into the analysis queue under a controlled
SOP to confirm package/sample integrity, appropriate shipping conditions (frozen
samples/dry ice, as appropriate), and individual confirmation of sample identity
against the shipping manifest and sample submission form and instructions.
Turn-around Time
Typical turn-around time is one to two weeks. If you have a critical time requirement,
we can often meet your need for no additional charge.
Read more about our commitment to prompt turn-around times.
Related Services
Asuragen’s Informatics
Team offers a broad range of analytical services to help you get the most
information from your data.
Catalog Numbers for Agilent Services
miRNA Expression Profiling
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CA0032
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QC, NanoDrop and TaqMan, Total RNA, for miRNA Applications
|
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CA0053
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Target Preparation, miRNA, Agilent miRNA V1 (Sanger 9.1) microarrays
|
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CA0056
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Array Processing and Scanning, Agilent V1 miRNA Arrays (Sanger 9.1)
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CA0077
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Target Preparation, miRNA, Agilent miRNA V2 & V3 microarray
|
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CA0078
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Array Processing and Scanning, Agilent miRNA V2 & V3 microarray
|
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CC0260
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Agilent Human miRNA Chip 9.1 (V1), 8 Arrays
|
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CC0270
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Agilent Rat miRNA Chip 10.1 (V2), 8 arrays
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CC0280
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Agilent Mouse miRNA Chip 10.1 (V2), 8 Arrays
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CC0290
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Agilent Human miRNA Chip 10.1 (V2), 8 Arrays
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miRNA Expression Data Analysis Options
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CS0010
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Data Analysis, Custom
|
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CS0020
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miRInform™ Data Delivery System for DiscovArray and Agilent miRNA Expression
|
aCGH
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CC0400
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Agilent Human 244A aCGH Array (1x244K)
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CC0440
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Agilent Human 105A aCGH Array (2x105K)
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CC0390
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Agilent Human 44K aCGH Array (4x44K)
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CC0410
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Agilent Mouse 244A aCGH Array (1x244K)
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CC0460
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Agilent Mouse 105A aCGH Array (2x105K)
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CC0420
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Agilent Mouse 44K aCGH Array (4x44K)
|
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CC0430
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Agilent Rat 244A aCGH Array (1x244K)
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CC0450
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Agilent Rat 105A aCGH Array (2x105K)
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