miRNA Expression Profiling Services
Asuragen Services provides a comprehensive solution to your miRNA analysis needs.
By using our rich heritage in RNA we are able to contribute a high level of expertise
to miRNA research. We have applied this experience to every platform we add to our
portfolio of offerings.
- Whole Genome
- Single gene or gene panels
Method and Staff Qualification
Asuragen employees have been developing and using miRNA profiling technologies since
2003. We have experience with a wide range of sample types, labeling methods, hybridization
and washing protocols, scanning platforms, normalization schemes, and data analysis.
Any new profiling technology or new operator at Asuragen Services must demonstrate
quality performance based on objective, quantitative measures before either is put
in service for client GLP projects. In the case of gene expression profiling, Asuragen
measures the following parameters against established benchmarks prior to release
of a new GLP protocol:
- Linear range, dynamic range, or range of quantitation
When a new team member joins the analysis team, they are provided hands-on training
with Asuragen-provided test materials. After their training period, data from a
qualification set of samples is evaluated on each operator before they are considered
“qualified” and allowed to process client samples. Operator metrics are tracked
daily, with appropriate contingencies in the event QC metrics fall below specified
levels. We are proud of our team members and they are proud of the work they do
on your studies.
No matter how robust the downstream processes, good data is dependent on sample
quality; consequently, accurate measurement of sample quality is essential before
deciding if a sample merits further analysis. All samples prepared by Asuragen Services
are routinely qualified by spectrophotometry with the NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer. Due to the degraded nature of RNA from FFPE tissues,
special quality control measurements are used in place of the Bioanalyzer. miRNA
quality cannot be interrogated directly in most samples because it represents 0.1
to 0.01% of the total RNA population, so Asuragen relies on conventional measures
of RNA quality as a proxy for miRNA quality and amount. However Asuragen’s procedures
on several platforms incorporate spiked-in controls and qRT-PCR measurements to
assess quality at various stages of processing.
Regardless of downstream application, detailed QC reports including nucleic acid
concentration, RNA integrity (RIN), electropherograms where applicable, and purity are
provided on each sample. For FFPE samples, concentration and QC pass/fail information
is provided. If a sample does not pass a recommended QC metric for analysis, you
will be contacted promptly to discuss possible solutions. These may include replacing
the sample or re-isolating the RNA, excluding the sample from further analysis,
or proceeding at-risk.
Asuragen’s rigorous procedures enable Asuragen to be the only service provider to
offer a Data Quality Guarantee:
If, for any reason, a qualified sample does not pass our process control specifications,
we will re-run the sample at no additional charge.
Read more about our
commitment to the best possible data quality, or read about our contributions to
in-process controls and
Sample Handling and Processing
All samples are received and entered into the analysis queue under a controlled
SOP to confirm package/sample integrity, appropriate shipping conditions (frozen
samples/dry ice, as appropriate), and individual confirmation of sample identity
against the shipping manifest and electronic
sample submission form and
Most projects are completed in one to two weeks. If you have a critical time requirement,
we can often meet your need for no additional charge.
Read more about our commitment to prompt turn-around times.
Additional Services Available
Requirements for miRNA Expression Profiling
Sample Input Requirements for the services listed above are found in the Sample Submission