Armored Technology™: Overview
Armored Technology provides:
- Protection of RNA targets from degradation – enabling stable RNA-based assay controls and standards
- Customizable RNA sequences – design controls specifically around your assay needs
- Non-infectious configuration – enabling safe handling and easy shipping
Armored RNA® is based on bacteriophage coat protein encapsulation of specific RNA targets to form pseudo-viral particles. The bacteriophage coat protein protects RNA transcripts from nuclease degradation and can stabilize RNA sequences.
Armored RNA® is designed for use as standards and controls in assays, in particular for use as positive controls or quantitative internal spiked controls for amplification and detection using RT-PCR.
Two versions of Armored RNA® are available to meet your specific needs:
Reference:
CR WalkerPeach & BL Pasloske. DNA Bacteriophage as Controls for Clinical Viral Testing. Clin Chem. 50(11)1970-71. 2004.
Armored RNA® Quant™ Applications:
- Quantitate samples against standard curve
- Internal Control to confirm negative results
- External Extraction Control to determine extraction efficiency
- External Control for direct detection of target
Armored RNA® Quant™ provides:
- Package target RNA of choice or ready-to-use products for popular targets
- Greater than 2000 bp insert target size
- Manufactured according to QSR guidelines
- Qualification using National Institute of Standards and Technology (NIST) traceable reference standard
- Custom fill and finish service available
Armored RNA® Quant™ technology stabilizes RNA transcripts from nuclease degradation by packaging them in a protective protein coat. The packaging of the RNA transcripts is performed in-vitro, enabling very tight control of RNA quality packaging. Due to the added stringency of this preparation, Armored RNA® Quant™ can be directly quantified via a primary phosphate standard certified by the National Institute of Standards and Technology (NIST).
Armored RNA® provides:
- Package target RNA of choice or ready-to-use catalog products for many applications
- 150 to 500 plus bp insert target size
Armored RNA® technology stabilizes RNA transcripts from nuclease degradation by packaging them in a protective protein coat. The packaging of a cloned RNA target sequence is driven off an inducible expression plasmid in host E coli.
