AmplideX® mPCR FMR1

  • Eliminates the need for Southern blot analysis
  • Accurately measures the methylation fraction for each FMR1 allele at higher resolution than Southern blot analysis
  • Provides increased sensitivity for the detection of lower abundance mosaic alleles*
  • Includes spike-in digestion and amplification controls to ensure high performance with every sample
  • Designed to complement the AmplideX FMR1 PCR Reagents*
  • Improves turnaround time by fivefold: 10 hours from DNA to data (as compared to 1 week for Southern blot)

Product Description

The AmplideX mPCR FMR1 Kit employs an innovative PCR-only approach for the detection of methylation status in the FMR1 gene. These reagents determine the extent of methylation of each individual allele in both male and female samples, and thus eliminate the need to run tedious and time consuming Southern blots.

AmplideX mPCR FMR1 Kit fig1 grey  AmplideX mPCR FMR1 Kit fig1.2

 

 

 

 

 

 

 

 

Figure 1. mPCR assay workflow

Product Description

A more informative and convenient alternative to Southern blot

Southern blotAmplideX mPCR FMR1 Kit fig2

AmplideX mPCR FMR1 Kit fig2.2

Figure 2. AmplideX® mPCR FMR1 Kit* provides intra-assay and allele-specific methylation quantification consistent with Southern blot analysis

 

AmplideX® mPCR FMR1 Kit* results correlate well with observed phenotype

AmplideX mPCR FMR1 Kit fig3

Observed phenotype: Low functioning female (65 IQ), anxiety, mood swings, and ADHD

Observed genotype: Full mutation allele identified using AmplideX PCR/CE FMR1 Kit and fully methylated status resolved using AmplideX mPCR FMR1 Kit. The results were concordant with Southern blot and consistent with the observed phenotype.

†Courtesy Rush University Medical Centre

Ordering

Product Name Number of Reactions Catalog Number
AmplideX PCR/CE FMR1 Control 24 UL 49513
AmplideX mPCR FMR1 Control 24 UL 49514
AmplideX PCR/CE FMR1 Reagents 100 49402
AmplideX PCR/CE FMR1 Kit 100 76008
AmplideX mPCR FMR1 Kit 24 49442
AmplideX PCR/CE FMR1 Reporter N/A 49576

T 1-877-777-1874; 512-681-5200
F 512-681-5202
E orders@asuragen.com

References

  1. A novel FMR1 PCR method for the routine detection of low abundance expanded alleles and full mutations in fragile X syndrome. Filipovic-Sadic et. al. Clin Chem. 2010 Mar; 56(3):399-408
  2. An information-rich CGG repeat primed PCR that detects the full range of fragile X expanded alleles and minimizes the need for southern blot analysis. Chen et. al. J Mol Diagn. 2010 Sep; 12(5):589-600
  3. High-resolution methylation polymerase chain reaction for fragile X analysis: evidence for novel FMR1 methylation patterns undetected in Southern blot analyses. Chen et. al. Genet Med. 2011 Jun; 13(6):528-38
  4. Evaluation of the human fragile X mental retardation 1 polymerase chain reaction reagents to amplify the FMR1 gene: testing in a clinical diagnostic laboratory. Nahhas et. al. Genet Test Mol Biomarkers. 2012 Mar; 16(3):187-92
  5. Performance evaluation of two methods using commercially available reagents for PCR-based detection of FMR1 mutation. Juusola et. al. J Mol Diagn. 2012 Sep;14(5):476-86

*For Research Use Only. Not for use in Diagnostic procedures.

AmplideX® mPCR FMR1

  • Eliminates the need for Southern blot analysis
  • Accurately measures the methylation fraction for each FMR1 allele at higher resolution than Southern blot analysis
  • Provides increased sensitivity for the detection of lower abundance mosaic alleles*
  • Includes spike-in digestion and amplification controls to ensure high performance with every sample
  • Designed to complement the AmplideX FMR1 PCR Reagents*
  • Improves turnaround time by fivefold: 10 hours from DNA to data (as compared to 1 week for Southern blot)

Product Description

The AmplideX mPCR FMR1 Kit employs an innovative PCR-only approach for the detection of methylation status in the FMR1 gene. These reagents determine the extent of methylation of each individual allele in both male and female samples, and thus eliminate the need to run tedious and time consuming Southern blots.

AmplideX mPCR FMR1 Kit fig1 grey  AmplideX mPCR FMR1 Kit fig1.2

 

 

 

 

 

 

 

 

Figure 1. mPCR assay workflow

Product Description

A more informative and convenient alternative to Southern blot

Southern blotAmplideX mPCR FMR1 Kit fig2

AmplideX mPCR FMR1 Kit fig2.2

Figure 2. AmplideX® mPCR FMR1 Kit* provides intra-assay and allele-specific methylation quantification consistent with Southern blot analysis

AmplideX® mPCR FMR1 Kit* results correlate well with observed phenotype

AmplideX mPCR FMR1 Kit fig3

Observed phenotype: Low functioning female (65 IQ), anxiety, mood swings, and ADHD

Observed genotype: Full mutation allele identified using AmplideX PCR/CE FMR1 Kit and fully methylated status resolved using AmplideX mPCR FMR1 Kit. The results were concordant with Southern blot and consistent with the observed phenotype.

†Courtesy Rush University Medical Centre

Ordering

Product Name Number of Reactions Catalog Number
AmplideX PCR/CE FMR1 Control 24 UL 49513
AmplideX mPCR FMR1 Control 24 UL 49514
AmplideX PCR/CE FMR1 Reagents 100 49402
AmplideX PCR/CE FMR1 Kit 100 76008
AmplideX mPCR FMR1 Kit 24 49442
AmplideX PCR/CE FMR1 Reporter N/A 49576

T 1-877-777-1874; 512-681-5200
F 512-681-5202
E orders@asuragen.com

References

  1. A novel FMR1 PCR method for the routine detection of low abundance expanded alleles and full mutations in fragile X syndrome. Filipovic-Sadic et. al. Clin Chem. 2010 Mar; 56(3):399-408
  2. An information-rich CGG repeat primed PCR that detects the full range of fragile X expanded alleles and minimizes the need for southern blot analysis. Chen et. al. J Mol Diagn. 2010 Sep; 12(5):589-600
  3. High-resolution methylation polymerase chain reaction for fragile X analysis: evidence for novel FMR1 methylation patterns undetected in Southern blot analyses. Chen et. al. Genet Med. 2011 Jun; 13(6):528-38
  4. Evaluation of the human fragile X mental retardation 1 polymerase chain reaction reagents to amplify the FMR1 gene: testing in a clinical diagnostic laboratory. Nahhas et. al. Genet Test Mol Biomarkers. 2012 Mar; 16(3):187-92
  5. Performance evaluation of two methods using commercially available reagents for PCR-based detection of FMR1 mutation. Juusola et. al. J Mol Diagn. 2012 Sep;14(5):476-86

*For Research Use Only. Not for use in Diagnostic procedures.

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