AB TaqMan® Gene Expression Services


Using the gold standard AB TaqMan® technology, Asuragen offers the most complete qRT-PCR service available.  Our validated procedures and optimized processes give you the quality to accurately quantify mRNA expression. Learn more about how we qualify our methods and staff.


Description of Services
Deliverables
Quality Control
Data Analysis
Turn-around Time
Additional Services Available
Available TaqMan Products
Catalog Numbers for Gene Expression qRT-PCR Services

Description of Services – mRNA, TLDA, and SNP

Workflow for all qRT-PCR services:

  1. Incoming total RNA QC (click here for sample prep services):

    1. Quantitation and estimation of RNA purity using NanoDrop spectrophotometer readings at 260 and 280 nm

    2. Assessment of RNA integrity using Agilent 2100 Bioanalyzer to measure RNA integrity number (RIN)

    3. See our Sample Submission Form and Shipping Instructions for recommended incoming sample QC values

  2. Reverse-transcription by random priming (default), oligo-dT priming, or gene specific priming

  3. Real-time detection via TaqMan primer & probe technology in triplicate

Workflow for all qPCR services:

  1. Incoming DNA QC by NanoDrop spectrophotometry

  2. Real-time detection via TaqMan primer & probe technology in triplicate

Sample Submission Form and Shipping Instructions.


Deliverables

As part of our TaqMan Gene Expression services, you will receive a complete data and experimental report containing:


mRNA — Relative Quantification

  • Raw CT values
  • ΔCT, or calibrator and samples against endogenous control
  • ΔΔCT, for calibrator and samples
  • Excel spreadsheet

SNP

  • Excel spreadsheet - Genotypes

TaqMan Low Density Array (TLDA)

  • Raw CT values
  • ΔCT, for calibrator and samples against endogenous control
  • ΔΔCT, for calibrator and samples
  • Excel spreadsheet
  • Excel spreadsheet – Genotypes (SNPs)

To ensure security, all information is provided in an electronic form on a DVD. Encryption is available on request. Data may also be delivered by FTP or e-mail.


Quality Control

During our processes, we monitor many QC checkpoints as recommended by the Applied Biosystems’ procedures. As much as possible, we utilize spike-in controls, in-process sample controls, and process or reagent batch controls. Should any sample-specific QC measure result in an “alert” or “fail” reading, we will contact you to discuss available options. These rigorous procedures enable Asuragen to be the only service provider to offer a Data Quality Guarantee:


  • If, for any reason, a qualified sample does not pass our process control specifications, we will re-run the sample at no additional charge.

Read more about our commitment to the best possible data quality, or read about our contributions to in-process controls and standardization.


Data Analysis

Asuragen is very experienced with qRT-PCR-based expression analysis. As part of the TaqMan services, we summarize the data and provide you with a complete data and experimental report. Relative quantitation is standard for TaqMan Gene Expression Assays. Absolute quantitation is available on a custom basis. Analytical assay validation services also are available.


Turn-around Time

Most projects are completed in two weeks or less.  If you have a critical time requirement, we can often meet your need for no additional charge. Read more about our commitment to prompt turn-around times.


Additional Services Available


Available TaqMan Products:

mRNA

TaqMan Gene Expression Assays and Arrays
Choose from over 750,000 pre-designed probe/primer sets.


TaqMan Gene Expression Assays

Species

Total Assays

Human

196,370

Mouse

174,469

Rat

150,001

Canine

22,616

Rhesus

11,788

Arabidopsis

97,232

C. elegans

92,284

Drosophila

40,520

 

 

Total*

785,280

TaqMan® Gene Expression Assays Product Guide (PDF, 1.0 MB)


SNP

All available assays


TLDA an Ideal Screening Technology
TaqMan Low Density Arrays are ideal for screening biomarkers and toxicology panels and for analyzing pathways, target classes, and complete disease sets. TLDA cards are pre-loaded for reliable performance and reproducible results.


Choose from one of the TLDA signature panels, gene sets or a completely customizable array.

Product Bulletin: TaqMan® Low Density Array: TaqMan Gene Expression Assays Products

More TLDA product literature


Catalog Numbers for Gene Expression qRT-PCR Services

CA0030 AB TaqMan Service for mRNA, individual assays, RT-PCR
CA0031  AB TaqMan Service for SNPs, PCR only
CA0035 AB TaqMan Assay Validation, mRNA
CA0046 AB TaqMan LDA card for mRNA, SNPs, or microRNA, PCR only
CA0047 RT for TaqMan mRNA card, random-primed
CA0037 QC, NanoDrop and TaqMan, Total RNA, for mRNA PCR
CS0016 Fee, TaqMan mRNA qRT-PCR Probe & Primer Design

*This test is performed pursuant to license arrangements with Roche Molecular Systems, Inc. and Applera Corporation.