AmplideX® Nanopore Carrier Plus Kit*
A Long-Range PCR & Long-Read Sequencing Solution for Carrier Screening Research
The AmplideX Nanopore Carrier Plus Kit interrogates eleven genes that contribute to high at-risk couple rates in carrier screening. The design utilizes four mixes across the 11 genes with modularity to allow any combination of testing.
With a single, flexible workflow designed to consolidate existing assays and methods, Carrier Plus can be used as a primary genetic analysis method or to complement existing NGS workflows for large panels. The use of long-read sequencing provides additional insights and reduces the need for reflex testing compared to traditional methods. The AmplideX One Reporter software further simplifies data analysis and reporting for a seamless, streamlined workflow.
Features & Benefits
One Streamlined Workflow
- Consolidated Workflow – Single, flexible workflow designed to consolidate existing assays and methods.
- Greater Insight – The use of long-read sequencing provides additional insight compared to existing methods (e.g. AGG interruptions in FMR1), reducing the need for reflex testing.
- Integrated Data Analysis – The AmplideX One Reporter software further simplifies data analysis and reporting with customized visuals for each variant type to assist genotype interpretation.
The design utilizes four mixes across the 11 genes with modularity to allow any combination of testing.
Kit Configuration – Eleven genes across four mixes
The AmplideX Nanopore Carrier Plus Kit: A Long-Range PCR & Long-Read Sequencing Solution for Carrier Screening Research
Workflow
One Workflow, Nine High-Prevalence Carrier Screening Conditions, 11 Genes
High level assay workflow for the AmplideX Nanopore Carrier Plus Kit
Hands-on time and instrument time for AmplideX Nanopore Carrier Plus Kit (24 samples run across Mix A, B, C)
Analytical Performance & Visual Outputs
Examples of visual outputs for various genes/mixes: Pre-Mutation (PM) sample with no AGG on PM allele (20(11AGG), 70 CGG). FMR1 output provides CGG sizing and AGG phase and location for each allele.
NA10797 – compound HET FIL/SEA sample: HBA1/2 fold change plot provides accurate copy number and identification of major deletion genotypes
NA14733 – 2 copies of CYP21A2 with one of the two copies being a CH-5 fusion: Resolution of CYP21A2/TNXB gene/pseudogene structure down to each exon provides clear visualization of the various changes in this region including phased SNVs.
Ordering Information
Product Details
Oxford Nanopore Technologies instruments for AmplideX Nanopore Carrier Plus Kit
MinION Mk1B MIN-101B
MinION MK1D MIN-101D
GridION GRD-MK1
About Our Partnership
Asuragen has leveraged its gold-standard PCR technology with Oxford Nanopore’s long-read DNA sequencing capabilities to develop the first sequencing system to identify the most challenging yet high prevalence carrier genes in a single, unified workstream that previously required multiple other methods.
Learn More About Our Partnership
Additional Resources
Videos
Watch Now: Long-Range PCR Meets Long-Read Sequencing: An Assay for 11 Hard-to-Decipher, High-Frequency Carrier Screening Associated Genes
Watch Now: Oxford Nanopore: Advancing Human Genetics & Cancer Research
Watch Now: Achieving Success with the AmplideX Nanopore Carrier Plus Kit: One Lab’s Experience
Watch Now: A Glimpse into AmplideX Nanopore Carrier Plus Kit in a Lab Setting
Scientific Posters
View Now: Verification of an Amplification Based Nanopore Sequencing Assay and Software to Genotype Complex, Clinically-Relevant Variants in 11 Hard-to-Decipher Genes with High Carrier Frequencies
View Now: Resolving Complex Genotypes in Residual Clinical Samples with Long Range-PCR and Nanopore Sequencing Assay
View Now: AMP 2024: Beta evaluation of AmplideX PCR enrichment technology with Nanopore long-read DNA sequencing of a panel of eleven challenging genes
Contact Us
Gain Deeper Insights
Disclaimer
*For Research Use Only. Not for use in diagnostic procedures.
Trademarks and registered trademarks are the property of their respective owners.
AmplideX® Nanopore Carrier Plus Kit*
A Long-Range PCR & Long-Read Sequencing Solution for Carrier Screening Research
The AmplideX Nanopore Carrier Plus Kit interrogates eleven genes that contribute to high at-risk couple rates in carrier screening. The design utilizes four mixes across the 11 genes with modularity to allow any combination of testing.
With a single, flexible workflow designed to consolidate existing assays and methods, Carrier Plus can be used as a primary genetic analysis method or to complement existing NGS workflows for large panels. The use of long-read sequencing provides additional insights and reduces the need for reflex testing compared to traditional methods. The AmplideX One Reporter software further simplifies data analysis and reporting for a seamless, streamlined workflow.
Features & Benefits
One Streamlined Workflow
- Consolidated Workflow – Single, flexible workflow designed to consolidate existing assays and methods.
- Greater Insight – The use of long-read sequencing provides additional insight compared to existing methods (e.g. AGG interruptions in FMR1), reducing the need for reflex testing.
- Integrated Data Analysis – The AmplideX One Reporter software further simplifies data analysis and reporting with customized visuals for each variant type to assist genotype interpretation.
The design utilizes four mixes across the 11 genes with modularity to allow any combination of testing.
Kit Configuration – Eleven genes across four mixes
The AmplideX Nanopore Carrier Plus Kit: A Long-Range PCR & Long-Read Sequencing Solution for Carrier Screening Research
Workflow
One Workflow, Nine High-Prevalence Carrier Screening Conditions, 11 Genes
High level assay workflow for the AmplideX Nanopore Carrier Plus Kit
Hands-on time and instrument time for AmplideX Nanopore Carrier Plus Kit (24 samples run across Mix A, B, C)
Analytical Performance & Visual Outputs
Examples of visual outputs for various genes/mixes: Pre-Mutation (PM) sample with no AGG on PM allele (20(11AGG), 70 CGG). FMR1 output provides CGG sizing and AGG phase and location for each allele.
NA10797 – compound HET FIL/SEA sample: HBA1/2 fold change plot provides accurate copy number and identification of major deletion genotypes
NA14733 – 2 copies of CYP21A2 with one of the two copies being a CH-5 fusion: Resolution of CYP21A2/TNXB gene/pseudogene structure down to each exon provides clear visualization of the various changes in this region including phased SNVs.
Ordering Information
Product Details
Oxford Nanopore Technologies instruments for AmplideX Nanopore Carrier Plus Kit
MinION Mk1B MIN-101B
MinION MK1D MIN-101D
GridION GRD-MK1
About Our Partnership
Asuragen has leveraged its gold-standard PCR technology with Oxford Nanopore’s long-read DNA sequencing capabilities to develop the first sequencing system to identify the most challenging yet high prevalence carrier genes in a single, unified workstream that previously required multiple other methods.
Learn More About Our Partnership
Additional Resources
Videos
Watch Now: Long-Range PCR Meets Long-Read Sequencing: An Assay for 11 Hard-to-Decipher, High-Frequency Carrier Screening Associated Genes
Watch Now: Oxford Nanopore: Advancing Human Genetics & Cancer Research
Watch Now: Achieving Success with the AmplideX Nanopore Carrier Plus Kit: One Lab’s Experience
Watch Now: A Glimpse into AmplideX Nanopore Carrier Plus Kit in a Lab Setting
Scientific Posters
View Now: Verification of an Amplification Based Nanopore Sequencing Assay and Software to Genotype Complex, Clinically-Relevant Variants in 11 Hard-to-Decipher Genes with High Carrier Frequencies
View Now: Resolving Complex Genotypes in Residual Clinical Samples with Long Range-PCR and Nanopore Sequencing Assay
View Now: AMP 2024: Beta evaluation of AmplideX PCR enrichment technology with Nanopore long-read DNA sequencing of a panel of eleven challenging genes