Features & Benefits

Reduced Complexity

• Broad coverage* using only PCR/CE workflow
• Similar testing process to AmplideX PCR/CE FMR1** and SMN1/2 Plus** kits eases implementation
• Streamlined data analysis via AmplideX Reporter software

Optimized Workflow

• Easy-to-use workflow with fewer hands on steps than most competitor assays
• Flexible use on widely installed CE equipment
• Less than 5 hours from DNA to data

Quality Results

• Built on the latest prevalence data to provide the best coverage* for all United States ethnicities
• Detects complex yet key CFTR mutations (STRs, SNPs, INDELs) and resolves zygosity
• Excellent concordance with other methods

Analytical Performance

Figure 1. Testing Workflow for AmplideX PCR/CE CFTR Kit.

Figure 2A. Ethnic diversity of 89K patients in CFTR2*** database.

Figure 2B. Ethnic diversity of 115K subjects in U.S. study.*

Table 1. Percent coverage of variants detected by commercially available kits based on frequencies observed in the CFTR2 database and the U.S. population.

Table 2A. Mutations in Cell Lines Detected by Prototype PCR/CE CFTR Kit and Sanger Sequencing.

Table 2B. Mutations in Clinical Samples Detected by Prototype PCR/CE CFTR Kit and Either xTAG® Cystic Fibrosis (CFTR) 60 Kit v2 or Sanger Sequencing.

Figure 3. AmplideX Reporter Software Output.

Additional Resources

VIDEO: “A Fast Track to Covering Your Bases: How the AmplideX® PCR/CE CFTR Kit Streamlines the Detection of Diverse Pathogenic Variants in Under Five Hours”

Does testing for more CFTR mutations yield greater coverage? Do your panels reflect your testing population’s diversity? This video evaluates the quality versus quantity of variants in CFTR mutation testing, relates this concept to the design, performance, and simplicity of the AmplideX® PCR/CE CFTR Kit**, and translates the benefits of this kit to the streamlined, scalable, and rapid detection of mutations across different populations.

WHITE PAPER: “Making Coverage Count: Screening for CFTR Mutations in Diverse Populations with Effective Variant Panels”

Many CFTR panels were designed using variant information from disease databases that are heavily skewed toward individuals with European ancestry. Recent studies, however, have shown that many CFTR carriers may be missed in ethnically-diverse populations*, including the US demographic. To address these coverage gaps, Asuragen scientists consulted recent, large-scale population studies in the development of the AmplideX PCR/CE CFTR Kit to ensure key CFTR variants could be detected across ethnic groups.

VIDEO: Answers for All: Addressing Coverage Gaps in Current CFTR testing

Cystic fibrosis screening tests should provide reliable answers for all people, but there are concerning gaps in CFTR coverage. We’re working to resolve this to ensure broad CFTR testing coverage for all. This video premiered at the 2021 ASHG annual meeting.

VIDEO: The AmplideX PCR/CE CFTR Kit Illustrated

POSTER: Quality Over Quantity: CFTR Panel Comparison (J. Larson, ASHG 2020)

CORPORATE WORKSHOP: Practical and Reliable CFTR Variant Detection for Diverse Populations with the AmplideX® PCR-CE CFTR Kit (AMP 2021)

In this online corporate workshop, we discuss the coverage gaps of current CFTR panels based on recent large-scale population studies and explain how this new data informed the design of the new AmplideX PCR/CE CFTR Kit (RUO) to provide broader coverage of the U.S. population in a streamlined, practical assay.

References

*Beauchamp et al, Genet Med. 2019 Nov;21(11):2569-2576z

**For Research Use Only. Not for use in Diagnostic procedures.

***The Clinical and Functional Translation of CFTR (CFTR2); available at http://cftr2.org

Features & Benefits

Reduced Complexity

• Broad coverage* using only PCR/CE workflow
• Similar testing process to AmplideX PCR/CE FMR1** and SMN1/2 Plus** kits eases implementation
• Streamlined data analysis via AmplideX Reporter software

Optimized Workflow

• Easy-to-use workflow with fewer hands on steps than most competitor assays
• Flexible use on widely installed CE equipment
• Less than 5 hours from DNA to data

Quality Results

• Built on the latest prevalence data to provide the best coverage* for all United States ethnicities
• Detects complex yet key CFTR mutations (STRs, SNPs, INDELs) and resolves zygosity
• Excellent concordance with other methods

Analytical Performance

Figure 1. Testing Workflow for AmplideX PCR/CE CFTR Kit.

Figure 2A. Ethnic diversity of 89K patients in CFTR2*** database.

Figure 2B. Ethnic diversity of 115K subjects in U.S. study.*

Table 1. Percent coverage of variants detected by commercially available kits based on frequencies observed in the CFTR2 database and the U.S. population.

Table 2A. Mutations in Cell Lines Detected by Prototype PCR/CE CFTR Kit and Sanger Sequencing.

Table 2B. Mutations in Clinical Samples Detected by Prototype PCR/CE CFTR Kit and Either xTAG® Cystic Fibrosis (CFTR) 60 Kit v2 or Sanger Sequencing.

Figure 3. AmplideX Reporter Software Output.

References

*Beauchamp et al, Genet Med. 2019 Nov;21(11):2569-2576z

**For Research Use Only. Not for use in Diagnostic procedures.

***The Clinical and Functional Translation of CFTR (CFTR2); available at http://cftr2.org