Features & Benefits

The QuantideX qPCR BCR-ABL minor Kit (RUO) delivers high performance through unmatched sensitivity and optimized laboratory efficiency.

Reduced Complexity
Ease-of-data analysis and reporting:

• Leverages the QuantideX^® qPCR BCR-ABL IS Kit workflow concept for streamlined implementation
• QuantideX qPCR Reporter provides automated calculation of BCR-ABL1/ABL1 % ratio and eliminates the need for manual calculation

Optimized Workflow
Valuable operator hands-on time has been significantly reduced through:

• Multiplexed design amplifies and detects both fusion and control gene in the same reaction
• All-inclusive reagents sourced and quality controlled together from a single vendor
• Pre-mixed reagents allow fewer pipetting steps in mastermix preparation

Quality Performance
Detecting BCR-ABL1 minor transcripts robustly and reliably with a highly sensitive assay:

• Ultra-sensitive Limit of Detection (LOD): Log reduction of 4.61 (0.0025% ratio)
• Increased analytical sensitivity without compromising analytical specificity: incorporates Limit of Blank (LOB) to prevent miscalling of non-leukemic low positives
• Armored RNA^®-based standards provide true RNA quantification

Analytical Characteristics

• Reproducible: Proven sensitivity based on rigorous testing criterion (Table 1)
• Precise: Minimal variability across entire dynamic range of BCR-ABL1/ABL1 % ratios (Table 2)
• Streamlined: Multiplexed design yields workflow and cost efficiencies (Figure 1)

Proven Sensitivity Based on Rigorous Testing Criterion
Table 1: LOD as determined by CLSI EP17-A2 guidelines by testing human RNA and cell line dilutions spanning lots, batch runs, days, operators and instruments.

Minimal Variability across Entire Dynamic Range

Table 2: Assay precision determined by testing 4 different log reduction (LR) levels in human RNA, using 2 operators and 8 runs for a total of 192 data points.

Multiplexed Design Yields Workflow and Cost Efficiency
  

Figure 1: Comparison of a plate layout for 8 sample run on Asuragen plate (left) and an alternate non-multiplex assay (right): 19 reactions for Asuragen setup vs. 52 reactions on a non-multiplex assay setup

Features & Benefits

The QuantideX qPCR BCR-ABL minor Kit marries improved efficiency with unprecedented sensitivity, empowering labs to assess the deepest molecular responses in patients harboring the minor breakpoint with the ease-of-use they’ve come to expect from Asuragen.

Reduced Complexity
Ease-of-data analysis and reporting:

• Shares a common workflow with the QuantideX^® qPCR BCR-ABL IS Kit to reduce training burden and streamline test implementation
• Included software provides automated calculation of BCR-ABL1/ABL1 % ratio and the ability to report BCR-ABL Major on both the International Scale (IS) and copy number^*

Optimized Workflow
Valuable operator hands-on time has been significantly reduced through:

• Multiplexed design amplifies and detects both fusion and control gene in the same reaction
• All necessary RT and qPCR reagents sourced and quality controlled together from a single vendor
• Pre-mixed reagents allow fewer pipetting steps in mastermix preparation

Quality Performance
Detecting BCR-ABL1 minor transcripts robustly and reliably with a highly sensitive assay:

• Ultra-sensitive Limit of Detection (LOD): Log reduction of 4.61 (0.0025% ratio)
• Increased analytical sensitivity without compromising analytical specificity: incorporates Limit of Blank (LOB) to prevent miscalling of non-leukemic low positives
• Armored RNA^®-based standards provide true RNA quantification

Analytical Characteristics

• Reproducible: Proven sensitivity based on rigorous testing criterion (Table 1)
• Precise: Minimal variability across entire dynamic range of BCR-ABL1/ABL1 % ratios (Table 2)
• Streamlined: Multiplexed design yields workflow and cost efficiencies (Figure 1)

Proven Sensitivity Based on Rigorous Testing Criterion
Table 1: LOD as determined by CLSI EP17-A2 guidelines by testing human RNA and cell line dilutions spanning lots, batch runs, days, operators and instruments.

Minimal Variability across Entire Dynamic Range

Table 2: Assay precision determined by testing 4 different log reduction (LR) levels in human RNA, using 2 operators and 8 runs for a total of 192 data points.

Multiplexed Design Yields Workflow and Cost Efficiency
  

Figure 1: Comparison of a plate layout for 8 sample run on Asuragen plate (left) and an alternate non-multiplex assay (right): 19 reactions for Asuragen setup vs. 52 reactions on a non-multiplex assay setup