QuantideX® qPCR PML-RARA Kit*

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Request more information about the QuantideX qPCR PML-RARA Kit

 

Sensitive and Scalable Detection of 3 PML-RARA Primary Fusion Transcripts

Bcr1, bcr2, and bcr3 aberrant fusions of the PML-RARA gene are hallmarks of acute promyelocytic leukemia (APL), a distinct and aggressive and time sensitive subtype of acute myeloid leukemia (AML). Quantification of PML-RARA fusion transcripts of translocation t(15;17) assesses tumor burden in studies for molecular remission in APL and is essential to accurate genotypic characterization of leukemic subtype. The QuantideX® qPCR PML-RARA Kit* offers sensitive and scalable detection of 3 PML-RARA aberrant fusions in a single, multiplex RT-qPCR reaction, with results in 5 hours.

Multiplex design reduces complexity and hands-on time

Visual comparison of a 96-well plate layout for an 8 run sample for the Muliplex Asuragen assay versus a Singleplex competitor assay

Figure 1. Comparison of plate layout for an 8 sample run between the (a) Multiplex Asuragen assay, with samples run in singlicate, resulting in only 19 reactions; and (b) a singleplex competitor assay, with samples run in duplicate, resulting in 52 reactions.

Total Hands-on Time of 2 hours and Instrument time of 3 hours for the QuantideX qPCR PML-RARA Kit

Figure 2. QuantideX qPCR PML-RARA kit* workflow

Download the QuantideX qPCR PML-RARA Kit Brochure

Resources

Prototype RT-qPCR PML::RARA Reagents and a Commercial PML-RARA Kit Yield Highly Correlated Results

Download the poster

CategoryDescription
PortfolioQuantideX | Oncology Monitoring
Assay CoverageMultiplexed design: target and reference genes detected in same well
• Long isoform (bcr1)
• Short isoform (bcr3)
• 95% Variable isoforms (bcr2); excludes very rare fusions involving ZBTB16, NPM1, NUMA1, TBLXR1, STAT5B or PRKAR1A genes
Reference GeneABL1
Kit Size60 reactions (similar to BCR-ABL; minimum batch size of 4 samples)
Sample TypeHigh quality, intact RNA (K₂EDTA)
ControlsPositive Controls: High Positive, Low Positive
Negative Control: ABL1 positive, PML-RARA negative
CalibratorsMultiplexed calibrators (4)
Assay Sensitivity1:10⁴ (4-log reduction)
PlatformABI 7500 Fast (Dx); post-launch expansion to QS5/7 Pro (Dx) w/BCR-ABL portfolio
RegulatoryRUO only

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Interested in becoming an Early Access Site?

Enrollment for early access sites will begin in Spring 2024.

 

*For Research Use Only. Not for use in diagnostic procedures.

QuantideX® qPCR PML-RARA Kit*

pml rara header image
Request more information about the QuantideX qPCR PML-RARA Kit

 

Sensitive and Scalable Detection of 3 PML-RARA Primary Fusion Transcripts

Bcr1, bcr2, and bcr3 aberrant fusions of the PML-RARA gene are hallmarks of acute promyelocytic leukemia (APL), a distinct and aggressive and time sensitive subtype of acute myeloid leukemia (AML). Quantification of PML-RARA fusion transcripts of translocation t(15;17) assesses tumor burden in studies for molecular remission in APL and is essential to accurate genotypic characterization of leukemic subtype. The QuantideX® qPCR PML-RARA Kit* offers sensitive and scalable detection of 3 PML-RARA aberrant fusions in a single, multiplex RT-qPCR reaction, with results in 5 hours.

Multiplex design reduces complexity and hands-on time

Visual comparison of a 96-well plate layout for an 8 run sample for the Muliplex Asuragen assay versus a Singleplex competitor assay

Figure 1. Comparison of plate layout for an 8 sample run between the (a) Multiplex Asuragen assay, with samples run in singlicate, resulting in only 19 reactions; and (b) a singleplex competitor assay, with samples run in duplicate, resulting in 52 reactions.

Total Hands-on Time of 2 hours and Instrument time of 3 hours for the QuantideX qPCR PML-RARA Kit

Figure 2. QuantideX qPCR PML-RARA kit* workflow

Download the QuantideX qPCR PML-RARA Kit Brochure

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