AmplideX® PCR/CE FMR1 Reagents
AmplideX PCR/CE FMR1 Reagents* are market-leading research tools for the detection of CGG repeats in the Fragile X Messenger Ribonucleoprotein 1 (FMR1) gene. These reagents provide a PCR-only approach based on Triplet Repeat Primed PCR (TP-PCR) design to reliably amplify and detect all alleles including Full Mutations.
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Our Product: AmplideX PCR/CE FMR1 Kit
Features & Benefits
AmplideX PCR/CE FMR1 Reagents* have created an easy-to-use, accessible, high performance method for laboratories to reliably analyze CGG repeats and detect interrupting AGG sequences in the FMR1 gene.
Reduced Complexity
Ease-of-analysis of the FMR1 gene has been simplified through:
- Implementation of proprietary PCR solution for amplifying GC-rich regions
- Automation of result calling using AmplideX PCR/CE FMR1 Analysis Module
Optimized Workflow
Valuable operator hands-on time has been significantly reduced through:
- Direct injection of PCR products (no PCR clean up) in to Capillary Electrophoresis platforms
- Decreased need for Southern blot analysis (up to 50 fold)
- End-to-end solution for FMR1 analysis including all necessary reagents and software
Quality Performance
Performing FMR1 Analysis with Greater Sensitivity and Accuracy:
- Detection of all allele expansions, including low abundance full mutation size mosaics with up to at least 1300 CGG repeats
- Up to 875 fold more sensitive than Southern blot1
- Resolution of female homozygous and heterozygous samples and indication of interrupting AGG sequences
- Proven performance as indicated by more than 30 peer reviewed publications
*For Research Use Only. Not for use in diagnostic procedures.
Product Description
Analytical Characteristics of AmplideX PCR/CE FMR1 Reagents*:
- Detects all alleles including low abundance full mutations (Figure 1)
- Accurately sizes any repeat up to 200 CGG repeats (Figure 2)
- Resolves female zygosity (Figure 3)
- Detects presence of AGG interruptions (Figure 4)
Figure 1: Amplification of Asuragen’s Methylation and Sensitivity Control which has a 5% full mutation in a background of 95% Normal
Figure 2. Female premutation sample with accurate sizing of Normal (30 CGG) and Pre mutation allele (56 CGG)
Figure 3: The difference in the “stutter” peak patterns of homozygous and heterozygous female provides a clear resolution of zygosity
Figure 4. Female Full Mutation sample with AGG interruptions as indicated by sudden decrease in peak heights of the “stutter” peak profile
Figure 5. Hands-on Time and Instrument time for the AmplideX PCR/CE FMR1 Reagents Kit
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Ordering Information
Product Name | Number of Reactions | Catalog Number |
---|---|---|
AmplideX PCR/CE FMR1 Control | 24 UL | 49513 |
AmplideX mPCR FMR1 Control | 24 UL | 49514 |
AmplideX PCR/CE FMR1 Reagents | 100 | 49402 |
AmplideX mPCR FMR1 Kit | 24 | 49442 |
AmplideX PCR/CE FMR1 Reporter | N/A | 49576 |
References
- Referenced in over 30 peer reviewed publications and used in over 200 laboratories, the AmplideX® PCR/CE FMR1 Reagents* are globally recognized as best-in-class for assessment of CGG repeats in the FMR1 gene.
AmplideX® PCR/CE FMR1 Kit
The AmplideX PCR/CE FMR1 Kit is an in vitro diagnostic (IVD) device with Class C Certification under the new European Union In Vitro Diagnostic Regulation (IVDR) for use in clinical laboratories for detection of the CGG repeats in the Fragile X Messenger Ribonucleoprotein 1 (FMR1) gene. This kit is intended to aid in the diagnosis of Fragile X Syndrome and Fragile X associated disorders, e.g. tremor and ataxia syndrome (FX-TAS) and primary ovarian insufficiency (FXPOI), through determination of CGG repeat length up to 200 CGG and detection of alleles greater than 200 CGG.
Order AmplideX PCR/CE FMR1 Kit
Our Product: AmplideX PCR/CE FMR1 Kit
Features & Benefits
The AmplideX PCR/CE FMR1 Kit is an all-inclusive kitted assay created to reliably analyze CGG repeats and detect interrupting AGG sequences in the FMR1 gene. Using a PCR-only approach based on Triplet Repeat Primed PCR (TP-PCR) design, the AmplideX PCR/CE FMR1 kit reliably amplifies and detects all alleles, including full mutations, in a streamlined workflow on widely available laboratory equipment.
Reduced Complexity
- End-to-end solution includes all necessary reagents
- Proprietary PCR solution reliably amplifies GC-rich regions, as indicated by more than 30 peer reviewed publications
- Automated result calling using AmplideX PCR/CE FMR1 Reporter Software
Optimized Workflow
- Direct injection of PCR products into Capillary Electrophoresis platforms reduces PCR clean up
- Decreased need for Southern blot analysis (up to 50-fold)
- Reduced hands-on time
Quality Performance
- Detection of all allele expansions, including low abundance full mutation size mosaics with up to at least 1300 CGG repeats
- Greater accuracy with up to 875-fold more sensitivity than traditional Southern blot techniques1
- Resolution of female homozygous and heterozygous samples and indication of interrupting AGG sequences
Product Description
Analytical Characteristics of AmplideX PCR/CE FMR1 Kit:
- Proven clinical accuracy compared to Southern Blot (Table 1)
- Detects all alleles including low abundance full mutations (Figure 1)
- Accurately sizes all alleles up to 200 CGG repeats (Figure 2)
- Resolves female zygosity (Figure 3)
- Detects presence of AGG interruptions (Figure 4)
Figure 1: Amplification of Asuragen’s Methylation and Sensitivity Control which has a 5% full mutation in a background of 95% Normal
Figure 2. Female Pre-mutation sample with accurate sizing of Normal (30 CGG) and Pre-mutation allele (56 CGG)
Figure 3: The difference in the “stutter” peak patterns of homozygous and heterozygous female provides a clear resolution of zygosity
Figure 4. Female Full Mutation sample with AGG interruptions as indicated by sudden decrease in peak heights of the “stutter” peak profile
Figure 5. Hands-on Time and Instrument time for the AmplideX PCR/CE FMR1 Reagents Kit
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Ordering Information
Product Name | Number of Reactions | Catalog Number |
---|---|---|
AmplideX PCR/CE FMR1 Control* | 24 UL | 49513 |
AmplideX mPCR FMR1 Control* | 24 UL | 49514 |
AmplideX PCR/CE FMR1 Reagents* | 100 | 49402 |
AmplideX PCR/CE FMR1 Kit | 100 | 76008 |
AmplideX mPCR FMR1 Kit* | 24 | 49442 |
References
- Referenced in over 30 peer reviewed publications and used in over 200 laboratories, the AmplideX® PCR/CE FMR1 Reagents* are globally recognized as best-in-class for assessment of CGG repeats in the FMR1 gene. *For Research Use Only. Not for use in diagnostic procedures.