Features & Benefits

Reduced Complexity

• Similar workflow for AmplideX PCR/CE FMR1*^† and DMPK*^† kits eases implementation and training.
• Scalable design enables high-throughput testing.
• Automated copy number reporting streamlines data analysis.

Optimized Workflow

• A simplified workflow with fewer hands on steps and no overnight hybridization.
• Fully kitted solution with fewer incoming QC steps.
• DNA-to-data in less than four hours with only 50 minutes of hands-on-time.

Quality Results

• Ability to differentiate between 0, 1, 2, 3 and ≥4 copies for both SMN1 and SMN2.
• High specificity for both SMN1 and SMN2 ensures reliable results.
• Accurate copy number resolution demonstrated through comparisons with multiple orthogonal methods.

*For Research Use Only. Not for use in Diagnostic procedures.
^†CE-IVD for US Export Only.

Analytical Characteristics

• Absolute assay specificity permits detection of SMN1–SMN2 chimeras, including those resulting from gene conversion (Figure 1)
• Strong assay concordance to comparator methods (Table 1)
• Significantly reduced workflow time compared to alternative method (Figure 2)

Figure 1: Clinical samples tested with the AmplideX PCR/CE SMN1/2 Kit have 100% agreement with previous characterization methods.

Figure 2: Detection and differentiation of SMN1 and SMN2 hybrid peaks.

Table 1: SMN1 and SMN2 copy number concordance of the AmplideX PCR/CE SMN1/2 Kit. 95% confidence intervals (CI) are indicated.

Figure 3: Workflow comparison of Asuragen AmplideX Technology and MLPA.

Analytical Characteristics