Features & Benefits

The QuantideX NGS RNA Lung Cancer Kit offers a unique NGS-in-a-Box™ solution that provides unprecedented NGS workflow efficiency and high sensitivity at low input amounts from precious FFPE NSCLC samples.

Reduced Complexity
Assay incorporates sample-to-data solutions in a unique NGS-in-a-Box™ configuration

• Single assay for a broad range of important NSCLC fusion targets
• All-inclusive reagents from reverse transcription to NGS-ready library
• Fully integrated data analysis pipeline

Optimized Workflow
Efficient workflow leads to lower operational costs and reduced hands-on and total turnaround time*

• Reduced labor vs. current commercially available kits
• Improved turnaround time enables higher throughput

^*Internal data on file.

Quality Performance
Highly sensitive assay with integrated, Sample-Aware™ bioinformatics software and built-in quality checks to minimize erroneous results and sample failures

• Highly sensitive detection of RNA-based fusions
• Low input (~20ng) of RNA from FFPE
• Sample-Aware™ bioinformatics analysis and sample quality control

Product Description

Relevant Content
A focus on only the most important lung cancer fusion genes, MET exon 14 skipping events, 3’/5′ imbalances and mRNA expression from multiple published data sources such as COSMIC, ClinicalTrials.gov, NCCN Guidelines, etc.

• 107 NSCLC-relevant fusions
• ALK, RET, ROS1, NTRK1 & PDGFRA 3’/5’ imbalances
• MET exon 14 skipping events (e13/e14; e14/e15; e13/e15)
• 23 mRNA targets, plus 3 endogenous control transcripts

A Fully Integrated Workflow
A unique NGS-in-a-Box™ configuration offers a simplified and fully integrated NGS workflow with cGMP-manufactured reagents, components and controls ready to run right out of the box.

Includes:

• RT-PCR reagents
• A unique RNA Lung Cancer Sample QC kit (QuantideX qPCR RNA Assay (Lung))
• Gene-specific PCR primers and Master Mix reagents
• Barcodes for sample multiplexing
• Library purification and quantification reagents
• Integrated data analysis and reporting software (QuantideX NGS Reporter)

Rapid & Efficient
Adopt and run NGS-based analysis with minimum investment of time and resources, regardless of NGS experience and infrastructure.

• Fully integrated workflow reduces complexity and ensures reliable reagent quality
• Push-button analysis & reporting makes bioinformatics easy and efficient, regardless of experience level
• Integrated kit reduces workflow time by over 50% than competing NGS panels

Performance Data
QuantideX NGS Assay is capable of detecting fusions and splice variants down to 1:100 cells. (a) Admixture of MET exon 14 positive cell line in the background of wild-type cells, and (b) fusion, and (c) 3’/5’ imbalance status for an admixture of EML4-ALK positive FFPE in the background of a negative FFPE.

Figure 3a

Figure 3b

Figure 3c

QuantideX® NGS Reporter

Push-button analytics & reporting suite – Set-up-and-go workflow designed for easy installation and implementation, right out of the box.

Runs directly on lab desktop – Install locally on a Windows® desktop computer. No prior bioinformatics experience or large server environments required.

Comprehensive reporting – Full bioinformatics and reporting of variants (SNVs, SNPs, Indels, fusions), and standard QC metrics are automatically calculated.

Integrates QuantideX RNA/DNA QC Assay – Sample-Aware™ bioinformatics with integrated functional template copy number analysis dramatically reduces false-call rates.

Seamless updates offered with new panel designs & variant annotation – Software updates allow new panels to be adopted without re-investing in analytics when the Asuragen QuantideX NGS product line is adopted.

Contact us to request the QuantideX Reporter

Features & Benefits

The QuantideX NGS RNA Lung Cancer Kit offers a unique NGS-in-a-Box™ solution that provides unprecedented NGS workflow efficiency and high sensitivity at low input amounts from precious FFPE NSCLC samples.

Reduced Complexity
Assay incorporates sample-to-data solutions in a unique NGS-in-a-Box™ configuration

• Single assay for a broad range of important NSCLC fusion targets
• All-inclusive reagents from reverse transcription to NGS-ready library
• Fully integrated data analysis pipeline

Optimized Workflow
Efficient workflow leads to lower operational costs and reduced hands-on and total turnaround time*

• Reduced labor vs. current commercially available kits
• Improved turnaround time enables higher throughput

^*Internal data on file.

Quality Performance
Highly sensitive assay with integrated, Sample-Aware™ bioinformatics software and built-in quality checks to minimize erroneous results and sample failures

• Highly sensitive detection of RNA-based fusions
• Low input (~20ng) of RNA from FFPE
• Sample-Aware™ bioinformatics analysis and sample quality control

Product Description

Relevant Content
A focus on only the most important lung cancer fusion genes, MET exon 14 skipping events, 3’/5′ imbalances and mRNA expression from multiple published data sources such as COSMIC, ClinicalTrials.gov, NCCN Guidelines, etc.

• 107 NSCLC-relevant fusions
• ALK, RET, ROS1, NTRK1 & PDGFRA 3’/5’ imbalances
• MET exon 14 skipping events (e13/e14; e14/e15; e13/e15)
• 23 mRNA targets, plus 3 endogenous control transcripts

A Fully Integrated Workflow
A unique NGS-in-a-Box™ configuration offers a simplified and fully integrated NGS workflow with cGMP-manufactured reagents, components and controls ready to run right out of the box.

Includes:

• RT-PCR reagents
• A unique RNA Lung Cancer Sample QC kit (QuantideX qPCR RNA Assay (Lung))
• Gene-specific PCR primers and Master Mix reagents
• Barcodes for sample multiplexing
• Library purification and quantification reagents
• Integrated data analysis and reporting software (QuantideX NGS Reporter)

Rapid & Efficient
Adopt and run NGS-based analysis with minimum investment of time and resources, regardless of NGS experience and infrastructure.

• Fully integrated workflow reduces complexity and ensures reliable reagent quality
• Push-button analysis & reporting makes bioinformatics easy and efficient, regardless of experience level
• Integrated kit reduces workflow time by over 50% than competing NGS panels

Performance Data
QuantideX NGS Assay is capable of detecting fusions and splice variants down to 1:100 cells. (a) Admixture of MET exon 14 positive cell line in the background of wild-type cells, and (b) fusion, and (c) 3’/5’ imbalance status for an admixture of EML4-ALK positive FFPE in the background of a negative FFPE.

Figure 3a

Figure 3b

Figure 3c

QuantideX® NGS Reporter

Push-button analytics & reporting suite – Set-up-and-go workflow designed for easy installation and implementation, right out of the box.

Runs directly on lab desktop – Install locally on a Windows® desktop computer. No prior bioinformatics experience or large server environments required.

Comprehensive reporting – Full bioinformatics and reporting of variants (SNVs, SNPs, Indels, fusions), and standard QC metrics are automatically calculated.

Integrates QuantideX RNA/DNA QC Assay – Sample-Aware™ bioinformatics with integrated functional template copy number analysis dramatically reduces false-call rates.

Seamless updates offered with new panel designs & variant annotation – Software updates allow new panels to be adopted without re-investing in analytics when the Asuragen QuantideX NGS product line is adopted.

Contact us to request the QuantideX Reporter